human ovarian cancer cell lines es 2  (ATCC)

Journal: Genome medicine

Article Title: Pan-cancer analysis identifies CD155 as a promising target for CAR-T cell therapy.

doi: 10.1186/s13073-025-01490-0

Figure Lengend Snippet: Fig. 6 PVRbbz CAR-T cells effectively killed multiple tumor cell lines even at low effector:target ratios and corroborated by cytokines release. A-L Cytotoxicity of PVRbbz CAR-T cells on PC3 (A and G), PANC1 (B and H), U20S (C and I), ES2 (D and J), Huh7 (E and K) and MM.1S (F and L) cells at the indicated effector:target (E:T) ratios after 12 h of co-culturing. Cell Lysis was determined by luciferase and relative IVIS picture was shown. Two independent experiments were performed. M and N The concentrations of TNF-α (M) and IFN-γ (N) in supernatants from cytotoxicity assays at 1:1 effector:target after co-culture with tumor cells for 12 h were measured by ELISA kits. Three independent experiments were performed. Significance was calculated by two-way ANOVA. All error bars represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Article Snippet: Prostate cancer cell lines PC3, C4-2, DU145, multiple myeloma MM.1S cell line, chronic myeloid leukemia K562 cell line, osteosarcoma cell lines U20S and 143B, ovarian cancer cell line ES2 and OVCAR8, pancreatic cancer cell line PANC1 and CAPAN-2, lung cancer cell line H292, colorectal carcinoma cell line HT29 and murine melanoma cell line B16 F10 were obtained from the American Type Culture Collection (ATCC).

Techniques: Lysis, Luciferase, Co-Culture Assay, Enzyme-linked Immunosorbent Assay